Custom antibody-oligo conjugates forCITE-seq and 10x Feature Barcoding
We conjugate the exact clone you have validated to a feature-barcode oligo that matches your 10x chemistry. QC-released for consistent signal, from 20 µg of antibody, in days.
Get a CITE-seq Conjugate QuoteThe catalog panel only gets you so far…
CITE-seq, cell hashing and 10x Feature Barcoding all rely on the same reagent: an antibody labeled with a barcoded oligo, known as an antibody-derived tag (ADT). For many studies, the ready-made version has two problems.
The clone you need isn't in the catalog. Ready-made panels come with a fixed list of markers and clones. If your target calls for a different clone, or an antibody you have already validated elsewhere, the fixed panel can't supply it.
Conjugating it yourself is hard to keep consistent. The number of oligos on each antibody affects the signal, and both too few and too many compromise it. Getting the degree of labeling right, and repeating it across a whole panel, is difficult to do reliably by hand.
Under- or over-labeling an antibody with oligos can cost you a marker across the entire run.
Your clone, your chemistry, QC-released
A custom ADT service for people building their own panels.
Your exact clone
Conjugate the antibody you have already validated, or a clone no commercial panel offers. Any host species and isotype, monoclonal or recombinant.
QC-released for signal
We set the number of oligos per antibody to the level that gives the best signal, and we measure it. Purity and free oligo are checked too, so one batch matches the next.
Days, from 20 µg
Test a candidate clone before you commit a large amount of antibody. We work at small scale, so a single custom order does not wait behind a production batch.
Matched to the 10x chemistry you already run
The capture sequence on your ADT has to match your kit. We make it in the format your kit uses, so it works with your existing library prep.
3′ Feature Barcoding
Single Cell 3′ v3.1 and GEM-X 3′ v4
Feature-barcode capture sequence, compatible with TotalSeq-B workflows.
5′ Immune Profiling
Single Cell 5′ / V(D)J
Capture sequence complementary to the 5′ template-switch oligo, compatible with TotalSeq-C workflows.
Poly-A capture
Original CITE-seq workflows
Oligo-dT-capturable handle, compatible with TotalSeq-A workflows.
Running a custom barcode set? Send us your oligo design and we will conjugate to it.
We control the degree of labeling, so your signal is reproducible
How many oligos sit on each antibody has a large effect on sensitivity. We set that number deliberately and confirm it before we ship.
One reagent, across the method family
The same barcoded antibody is used for surface, nuclear, chromatin and multi-modal single-cell readouts. Tell us the method and we will make the conjugate to suit.
CITE-seq
Surface protein plus transcriptome from the same cell, read out alongside your gene expression library.
Cell hashing & multiplexing
Hashtag oligos (HTOs) against ubiquitous markers to pool samples in one run and demultiplex by tag.
inCITE-seq
Intranuclear and transcription-factor profiling in fixed, permeabilized nuclei, where conjugate behavior is especially demanding.
ASAP-seq & ECCITE-seq
Protein with chromatin accessibility, or five-modality readouts including clonotype and CRISPR guides.
How a custom ADT project runs
Define the tag
Tell us your antibody, your chemistry (3′, 5′ or poly-A), and your barcode design. We set scope, capture format, degree of labeling and QC release criteria.
Conjugation & QC
We conjugate and purify your ADT at µg scale, then validate degree of labeling, purity and free oligo against the agreed spec.
Dispatch & re-order
Your QC-released tag ships assay-ready with a release report. Re-orders use the same process and criteria, so panels stay consistent run to run.
Who orders custom ADTs from us
- Single-cell immunologists building panels with clones no catalog carries
- Core facilities supplying multiple PIs who need consistent, schedulable batches
- Multi-omics and spatial groups who have outgrown off-the-shelf ADT panels
- Labs running fixed-nuclei or intranuclear workflows, where conjugate quality matters most
- Method developers scoping a reagent before scaling a study
Get the clone your panel actually needs
Tell us your antibody, your chemistry and your barcode, and we will scope a QC-released ADT that works in your run.
Request a CITE-seq conjugate quote
Share your antibody, target method and chemistry (3′, 5′ or poly-A) and we will reply with scope, timeline and pricing.