Custom DNA-barcoded antibodies forspatial and multiplexed imaging
Send us the clone your panel is missing and the barcode sequence your platform expects. We make the oligo, conjugate it, tune the degree of labeling and QC-release the batch. From 20 µg of antibody, in days.
Get a Conjugate QuoteYour panel stops at the edge of somebody else's catalog
Every multiplexed imaging method runs on the same reagent: an antibody carrying a DNA barcode. The method scales beautifully until you need a marker that the platform's catalog does not carry.
The clone you need was never barcoded. A rare target, a species the vendor skipped, or the specific clone your lab has already validated on your tissue. The catalog cannot supply it, and swapping to whatever clone is available means revalidating from scratch.
Conjugating it yourself is hard to keep consistent. The number of oligos on each antibody drives both signal and background. Too few and the marker disappears in a crowded panel, too many and you lose affinity and pick up non-specific binding. Hitting the same number twice with a bench kit is the difficult part.
One badly labeled antibody costs you a marker across every region of every section in the run.
Your clone, your barcode sequence, QC-released
A custom conjugation service for people building their own multiplexed panels.
Your exact clone
Conjugate the antibody you have already validated on your tissue, or a clone no imaging panel offers. Any host species and isotype, monoclonal or recombinant.
Degree of labeling you can hold
Imaging rewards light labeling. We can work down to 1 to 4 oligos per antibody, set it deliberately, and measure it on every batch so one order matches the next.
Days, from 20 µg
Screen a candidate clone on a section before you commit a whole vial. We work at microgram scale, so a single custom probe does not queue behind a production batch.
Built to the sequence your method runs on
We are barcode-agnostic. You tell us the sequence your workflow expects, and we build the conjugate around it.
Cyclic immunofluorescence panels
Long barcodes in the 40 to 70 nt range for iterative hybridize-and-image workflows, including CODEX and PhenoCycler style panels built on published sequences.
DNA-PAINT & Exchange-PAINT
Short docking strands, typically 10 to 21 nt, for single-molecule localization and sequential target exchange.
Immuno-SABER
Primer and hybridization handles for branched signal amplification, conjugated to the clone you specify.
CyCIF & open cyclic-IF
Generic hybridizable oligos for open cyclic imaging workflows, where you control the barcode design end to end.
Running a method that is not listed? If you can give us the sequence, we can usually build the conjugate. Ask us.
You supply the sequence. We supply everything after it.
You do not need to order, modify or ship oligos. Send the barcode sequence your platform expects and we take it from there: we make the oligo to that sequence, build in the linker chemistry, conjugate it to your antibody, remove unbound oligo and release the batch against an agreed spec.
That means no purchase order for a modified oligo, no cold-chain shipment of a reagent you then have to QC, and no guessing which modification our chemistry wants. It also means the conjugation chemistry stays matched to the oligo, which is the part that usually goes wrong when the two are sourced separately.
Sequences for published imaging panels are widely available in the primary literature, and many groups design their own. You are responsible for having the right to use the sequence you send us.
What we can build
The working envelope, so you can tell in a minute whether we fit your panel.
- Oligo length
- Roughly 10 to 70 nt, covering short docking strands through long imaging barcodes.
- Attachment point
- 5′ end, via a C6 spacer and maleimide chemistry.
- Degree of labeling
- Tuned to your assay, down to 1 to 4 oligos per antibody, and measured on every batch.
- Purification
- Unbound oligo removed as standard, so free barcode does not compete in your hybridization.
- Starting material
- From 20 µg of antibody. Buffer and carrier constraints checked before we start.
- Turnaround
- Days rather than weeks for a single custom probe.
Unusual oligo modifications are assessed case by case. Tell us the full design in your enquiry and we will confirm what is feasible before quoting.
We control the degree of labeling, so your signal is reproducible
In a multiplexed panel every marker has to sit in the same dynamic range. That starts with knowing how many oligos are on each antibody, and being able to hit that number again on the re-order.
How a custom imaging probe runs
Send the clone and the sequence
Tell us the antibody, the barcode sequence your method needs, and the panel it has to sit in. We agree scope, degree of labeling and QC release criteria before any material moves.
Synthesis, conjugation and QC
We make the modified oligo, conjugate at microgram scale, remove unbound oligo, then confirm degree of labeling and purity against the agreed spec.
Stain, then re-order to the same spec
Your probe ships assay-ready with a release report. Re-orders run to the same criteria, so a panel you validated this year still behaves next year.
Who orders custom imaging conjugates from us
- Spatial biology groups adding markers their platform panel does not carry
- Imaging core facilities supplying several PIs who need batches that match
- Super-resolution labs building their own DNA-PAINT docking strand sets
- Groups running open cyclic-IF workflows with barcodes they designed themselves
- Labs currently conjugating with bench kits and losing time to batch variability
- Method developers screening a clone before committing it to a full study
Get the marker your panel is missing
Tell us the clone and the barcode sequence, and we will come back with scope, timeline and pricing.
Request a spatial conjugate quote
Share your antibody, the imaging method it has to work in, and the barcode sequence your platform expects. We will reply with scope, timeline and pricing.