Antibodies

Anti-CDKN1A/p21 Antibody-Oligo Conjugate

SKU: aoc1030

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Assay

Immunofluorescence Oligossee protocol

IF1 Oligo Details

5'
AATATGGAATTCGTCCGAGCCCGTCAAG
3'

View Paper

Accessory oligo is complementary to only the ab-conjugated oligo

Anti-CDKN1A/p21 Antibody-IF1

Antibody Target

Target
Cyclin-dependent kinase inhibitor 1
UniProt Number
P38936
Additional Target Information
This antibody also binds Cyclin-dependent kinase inhibitor 1, Uniprot:P39689.
Target Description
Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes.
Molecular Weight
18 kDa

Antibody Characteristics

Clonality
Monoclonal
Application
WBICCELISA
Isotype
IgG
Form
Liquid
Immunogen
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Immunogen Species
Human/Mouse
Host Species
Rabbit
Species Reactivity
HumanMouse