Conjugation Chemistry & Methods
Site-directed antibody conjugation methods 1: application of transglutaminase in the production of antibody-oligo conjugates
Transglutaminase catalyses the formation of an isopeptide bond between a lysine (K) residue and the γ-carboxamide group of a glutamine (Q) residue. The microbial (mTGase) rather than the eukaryotic enzyme tends to be used.