Overview from a practical standpoint, three factors dominate conjugate behaviour: where attachments occur, how many attachments are installed (the degree of labeling (DoL) and the distribution of drug-antibody ratios (DARs).
Many existing methods for conjugating oligonucleotides to biomolecules suffer from several drawbacks, including a lack of site-specificity (hence formation of heterogeneous mixtures), poor chemical or hydrolytic stability.
Transglutaminase catalyses the formation of an isopeptide bond between a lysine (K) residue and the γ-carboxamide group of a glutamine (Q) residue. The microbial (mTGase) rather than the eukaryotic enzyme tends to be used.
Conventional microarrays are made by adsorbing antibodies onto hydrophobic or chemically reactive solid surfaces, which can lead to denaturation, random orientation, loss of binding activity and reduced assay sensitivity.
Tags that appear alongside “Site-Specific” in articles.