Proximity Ligation Assay (PLA) is a highly sensitive molecular biology technique designed to detect and visualize protein-protein interactions directly in cells and tissues.
Complete Proximity Ligation Assay (PLA) protocol for immunofluorescence format. Step-by-step guide including cell fixation, AOC binding, ligation, rolling circle amplification, and troubleshooting.
Sprox-seq combines proximity ligation with spatial platforms to map protein-protein interactions in tissue. Profile 32 proteins and 528 pairwise interactions with preserved spatial coordinates.
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