Immuno-droplet digital PCR uses antibody-DNA conjugates to convert single-EV protein binding into amplifiable DNA barcodes, enabling multiplexed single-vesicle protein profiling with a 38 EV/µL limit of detection and direct measurement of PD-L1 heterogeneity in tumor-derived EVs.
NULISA redesigns paired antibody-oligonucleotide conjugates so the DNA both purifies intact immunocomplexes and generates a ligated reporter. The dual capture-and-release workflow suppresses background to reach attomolar sensitivity in single-plex and broad 200-plex multiplexing in NULISAseq.
Complete Proximity Ligation Assay (PLA) protocol for immunofluorescence format. Step-by-step guide including cell fixation, AOC binding, ligation, rolling circle amplification, and troubleshooting.
Complete immunofluorescence protocol for FFPE tissue staining using antibody-oligo conjugates. Step-by-step guide including antigen retrieval, blocking, staining with imaging strands, and troubleshooting.
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